Validation of the Key Active Ingredients and Anti-Inflammatory and Ana | DDDT - Dove Medical Press

Mei-Xiang Yu,^1,* Xiao-Qin Ma,^1,* Xin Song,² Yong-Mei Huang,³ Hui-Ting Jiang,¹ Jing Wang,¹ Wan-Hua Yang^1,4

¹Department of Pharmacy, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, People’s Republic of China; ²South Campus, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 201100, People’s Republic of China; ³Jinshan Hospital, Shanghai Fudan University School of Medicine, Shanghai 201508, People’s Republic of China; ⁴Department of Pharmacy, Ruijin Hospital North Affiliated to the Shanghai Jiao Tong University Medical School, Shanghai 201801, People’s Republic of China

*These authors contributed equally to this work

Correspondence: Wan-Hua Yang Email yangwanhuaxy@163.com

Background: Shenjin Huoxue Mixture (SHM), a classic traditional herb mixture has shown significant clinical efficacy against osteoarthritis (OA). Our previous experimental study has confirmed its anti–inflammatory and analgesic effect on acute soft tissue injury in rats, with the compound of glycyrrhizinate in SHM identified and the content of paeoniflorin in SHM determined by high-performance liquid chromatography (HPLC). However, the components and its pharmacological mechanisms of SHM against OA have not been systematically elucidated yet. Thus this study aimed to predict the key active ingredients and potential pharmacological mechanisms of SHM in the treatment of OA by network pharmacology approach and thin-layer chromatography (TLC) validation.
Methods: The active ingredients of SHM and their targets, as well as OA-related targets, were identified from databases. The key active ingredients were defined and ranked by the number of articles retrieved in PubMed using the keyword “(the active ingredients [Title/Abstract]) AND Osteoarthritis[Title/Abstract] ”, and validated partially by TLC. The pharmacological mechanisms of SHM against OA were displayed by GO term and Reactome pathway enrichment analysis with Discovery Studio 3.0 software docking to testing the reliability.
Results: Finally, 16 key active ingredients were identified and ranked, including quercetin validated through TLC. Inflammatory response, IL-6 signaling pathway and toll-like receptor (TLR) cascades pathway were predicted as the main pharmacological mechanisms of SHM against OA. Especially, 12 out of 16 key active ingredients, including validated quercetin, were well docked to IL-6 proteins.
Conclusion: Our results confirmed the anti–inflammatory and analgesic effect of SHM against OA through multiple components, multiple targets and multiple pathways, which revealed the theoretical basis of SHM against OA and may provide a new drug option for treating OA.

Keywords: Shenjin Huoxue Mixture, osteoarthritis, active ingredients, pharmacological mechanisms, network pharmacology, thin-layer chromatography

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Let's block ads! (Why?)

"ingredients" - Google News
March 16, 2020 at 04:14AM
https://ift.tt/2waHQph

Validation of the Key Active Ingredients and Anti-Inflammatory and Ana | DDDT - Dove Medical Press
"ingredients" - Google News
https://ift.tt/2Qstat1
Shoes Man Tutorial
Pos News Update
Meme Update
Korean Entertainment News
Japan News Update

Bagikan Berita Ini